Journal: Journal of Advanced Research

Article Title: An injectable nano-hydroxyapatite-incorporated hydrogel with sustained release of Notoginsenoside R1 enhances bone regeneration by promoting angiogenesis through Notch1/Akt signaling

doi: 10.1016/j.jare.2025.05.025

Figure Lengend Snippet: (A) The angiogenic-related gene expression quantified by qRT-PCR at day 4, day 7 and day 10. (B) The angiogenic proteins of VEGF and VEGFR-2 analyzed by western blot. (C)semiquantitative analysis of VEGF and VEGFR-2 protein expression. (D) The critical markers Notch1, Hes1 involved in the Notch 1 pathway analyzed by western blot and their semiquantitative analysis (E).(F)Western blot and semiquantitative analysis (G) of Akt protein expression.(H)The gene expression of Akt and VEGF under NGR1 stimulation with Notch 1 inhibition. (I) The gene expression of Notch 1 and VEGF under NGR1 stimulation with Akt inhibition. (J) Western blot and semiquantitative analysis (K) of Akt protein expression induced by NGR1 under Notch 1 inhibition. *p < 0.05, **p < 0.01, ***p < 0.001, ns, not significant.

Article Snippet: Subsequently, Notch 1 inhibitor (HY-1302, MCE, USA) and Akt inhibitor (HY-10358, MCE, USA) were applied to evaluate their effects on angiogenic-related gene expression.

Techniques: Gene Expression, Quantitative RT-PCR, Western Blot, Expressing, Inhibition

Journal: bioRxiv

Article Title: Graded Notch Signaling Functions as a Rheostat of Lineage Plasticity and Therapy Resistance in Prostate Cancer

doi: 10.1101/2025.11.23.690056

Figure Lengend Snippet: (A-D) Box and scatter plots showing bulk RNA-sequencing data for key Notch pathway genes across a panel of human prostate cancer cell lines (LNCaP, LNCaP-abl, C4-2, C4-2B, VCaP, 22RV1, PC3, DU145, H660, LASCPC1, LNCaP_42D). (A-B) NOTCH1 and NOTCH2 Gene Expression (receptor). (C) HES1 Gene Expression (downstream effector). (D) DLL3 Gene Expression (ligand). (E) Western blot analysis confirming the protein expression of NOTCH2, DLL3, and HES1 in selected cell lines (LNCaP/AR, PC3, gTP53/RB1-R, and LASCPC1), corroborating the gene expression trends observed in the bulk RNA-seq data. Actin serves as a loading control.

Article Snippet: A pool of siRNAs targeting human NOTCH1, NOTCH2, HES1, and a non-targeting control pool were used (MedChemExpress; HY-RS09445, HY-RS09448, HY-RS06134).

Techniques: RNA Sequencing, Gene Expression, Western Blot, Expressing, Control

Journal: bioRxiv

Article Title: Graded Notch Signaling Functions as a Rheostat of Lineage Plasticity and Therapy Resistance in Prostate Cancer

doi: 10.1101/2025.11.23.690056

Figure Lengend Snippet: (A, B) Relative gene expression of Notch pathway components and lineage markers in enzalutamide-resistant cell lines, (A) gTP53/RB1-R and (B) PC3, after siRNA knockdown of specific genes ( NOTCH1, NOTCH2, HES1 ) compared to non-targeting control (NC). Significance is denoted as *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: A pool of siRNAs targeting human NOTCH1, NOTCH2, HES1, and a non-targeting control pool were used (MedChemExpress; HY-RS09445, HY-RS09448, HY-RS06134).

Techniques: Gene Expression, Knockdown, Control